Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Study of different proteoglycan salts].

S M Bychkov, V N Bogatov, S A Kuz'mina

    Biulleten' Eksperimental'Noi Biologii I Meditsiny
    |September 1, 1981
    PubMed
    Summary

    Researchers developed methods to prepare hyaluronic acid (HA) and protein-chondroitin-keratan sulfate (PCKS) and their salts. Infrared spectroscopy revealed intermolecular hydrogen bonds in HA and PCKS macrocomplexes, crucial for their structure.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Proteoglycans and cells].

    Biulleten' eksperimental'noi biologii i meditsiny·1996
    Same author

    [Function of carboxyl and sulfate groups in proteoglycans].

    Biulleten' eksperimental'noi biologii i meditsiny·1995
    Same author

    [The role of protein components of proteoglycans in steric exclusion of cells].

    Biulleten' eksperimental'noi biologii i meditsiny·1994
    Same author

    [Erythrocyte aggregation in the blood in different states of the animal organism].

    Biulleten' eksperimental'noi biologii i meditsiny·1993
    Same author

    [Action of proteoglycans on erythrocytes in circulating blood].

    Biulleten' eksperimental'noi biologii i meditsiny·1993
    Same author

    [The mechanism of the steric exclusion of cells brought about by proteoglycans].

    Biulleten' eksperimental'noi biologii i meditsiny·1992

    Area of Science:

    • Biochemistry
    • Materials Science

    Background:

    • Hyaluronic acid (HA) and protein-chondroitin-keratan sulfate (PCKS) are complex glycosaminoglycans with significant biological roles.
    • Understanding their aggregation properties and salt forms is crucial for applications in medicine and biomaterials.

    Purpose of the Study:

    • To develop reliable methods for preparing pure hyaluronic acid (HA) and non-aggregating protein-chondroitin-keratan sulfate (PCKS).
    • To synthesize various salt forms (Na+, K+, Ca2+, Mg2+; acid and neutral) of HA and PCKS.
    • To investigate the structural basis of macrocomplex formation in these molecules using infrared spectroscopy.

    Main Methods:

    • Preparation of free HA and non-aggregating PCKS fractions.
    • Synthesis of diverse cationic salts of HA and PCKS.
    • Infrared (IR) spectroscopy analysis to identify intermolecular hydrogen bonding.

    Main Results:

    • Successful preparation of free HA and non-aggregating PCKS.
    • Characterization of various salt forms of HA and PCKS.
    • Infrared spectroscopy confirmed intermolecular hydrogen bonds in HA and PCKS macrocomplexes and PCKS acid salts.
    • HA salts did not appear to form macrocomplexes via intermolecular hydrogen bonds.

    Conclusions:

    • Novel methods for preparing specific HA and PCKS fractions and their salts have been established.
    • Intermolecular hydrogen bonds play a key role in the macrocomplex formation of PCKS and its acid salts.
    • HA salts exhibit different aggregation behavior compared to PCKS, suggesting distinct structural mechanisms.

    Related Experiment Videos