[Translation of mRNA extracted from human placenta in a wheat germ cell free system]

The mRNA were isolated from total RNA extracted from placenta by affinity chromatography on poly U Sepharose 4 B and were tested with wheat germ cell free system. The neosynthesized hPL is isolated by specific immunoprecipitation using 2 antibodies from the CEA (hPLK1 and hPLK3). It represented 7% of the total radioactive synthesized proteins. Two components were separated by gel electrophoresis. One is the natives hormone while the other which is the major component, migrated with a molecular weight of 25 000. These results are in accordance with a functionally cell free system which could be used study the non histone protein's role in the hPL synthesis.