Ethionine alters the urinary excretion of N1-methylnicotinamide

Nicotinamide is methylated via S-adenosylmethionine (AdoMet) and excreted in the urine as N1-methylnicotinamide. Since S-adenosylethionine (AdoEth) inhibits nicotinamide methyltransferase, ethionine (0.75 mg/g body wt) was given to rats and its effect on the urinary excretion of N1-methylnicotinamide was examined. Nicotinamide (0.5 mg/g body wt) gave a 13.6-fold increase in the 24-h urine concentration of N1-methylnicotinamide. However, ethionine given 4 h prior to nicotinamide completely prevented this increase by nicotinamide. It appears at ethionine via AdoEth inhibits nicotinamide methyltransferase in vivo and lowers the urine level of N1-methylnicotinamide.