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Related Experiment Videos

New methods in coagulation.

D A Triplett

    Critical Reviews in Clinical Laboratory Sciences
    |January 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Synthetic substrates and immunologic assays are revolutionizing coagulation diagnostics. These advancements enable precise quantitation of enzymes and inhibitors, improving the diagnosis of hereditary coagulation defects and carrier states like hemophilia A.

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    Area of Science:

    • Biochemistry
    • Hematology
    • Diagnostic Laboratory Medicine

    Background:

    • Advances in molecular biochemistry of coagulation proteins.
    • Development of synthetic substrates for enzyme assays.
    • Introduction of immunologic procedures for hereditary coagulation defects.

    Purpose of the Study:

    • To describe novel synthetic substrates for coagulation assays.
    • To highlight the role of immunologic procedures in diagnosing coagulation disorders.
    • To discuss advancements in quantifying enzymes, inhibitors, and platelet-specific proteins.

    Main Methods:

    • Oligopeptide substrates with chromophore/fluorophore groups for serine protease assays.
    • Immunologic assays correlated with coagulation assays and clinical data.

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  • Ristocetin cofactor assays with Factor VIII antigens and platelet-specific protein assays (e.g., ELISA, radioimmunoassay).

    • Main Results:

    • Synthetic substrates offer precise quantitation, standardization, and precision in coagulation assays.
    • Immunologic assays aid in identifying subgroups of hereditary deficiencies and carrier states (e.g., hemophilia A).
    • Combined assays improve diagnosis of von Willebrand's disease and provide evidence of in vivo platelet activation.

    Conclusions:

    • Synthetic substrates and immunologic assays are transforming coagulation diagnostics.
    • These methods enhance the precision and scope of laboratory testing for coagulation disorders.
    • Future research may correlate platelet-specific proteins with platelet kinetics.