Histochemical fibre-type profile in the human masseter muscle

A histochemical characterization of the masseter muscle was performed on biopsy samples of dentate subjects with normal occlusion. There was a continuum of ranges of oxidative and glycolytic capacities of the masseter muscle fibres. Besides the lightly-stained type I and the darkly-stained type II fibres, fibres with intermediate staining reactions for standard ATPase at pH 9.4, IM fibres, were seen in all biopsy samples. IM fibres had some staining characteristics in common with type I, i.e. the reaction for NADH-TR and for ATPase after preincubation at pH 4.6 and 4.2. Like type II fibres they showed strong reaction for menadione-linked alpha-glycerophosphate dehydrogenase and for phosphorylase. The ATPase reaction after preincubation at pH 4.6 did not generally reveal subtypes of type II. It is concluded that the masseter muscle in normal human subjects has a very special fibre composition, with ATPase-IM fibres being a part of the normal fibre population.