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Altered immunoregulatory function in long-term renal allograft recipients
In order to determine immunoregulatory lymphocyte subsets in patients with long surviving renal allografts, a study utilising functionally characterised monoclonal antibodies with analysis by flow cytometry using a Fluorescence Activated Cell Sorter was carried out. Cells from 35 patients with allograft survival from one to 15 years were analysed with monoclonal antibodies for the following markers: inducer-helper, cytotoxic-suppressor, monocytes, Ia, IgG and IgM. Control groups consisted of long-term dialysis patients and a group of normal individuals. The results show a strikingly significant difference between the long-term allograft recipients and the control population in terms of the inversion of the normal inducer-helper to cytotoxic-suppressor ratio. Additionally, there were significant differences in the number of Ia+ cells in long-term transplant patients and dialysis patients compared with normals. No impressive differences were found in the numbers of Ig bearing cells. Thus the long-term allograft state is a clear example of an overabundance of cellular suppression and may explain many of the general phenomena seen in surviving recipients.
In order to determine immunoregulatory lymphocyte subsets in patients with long surviving renal allografts, a study utilising functionally characterised monoclonal antibodies with analysis by flow cytometry using a Fluorescence Activated Cell Sorter was carried out. Cells from 35 patients with allograft survival from one to 15 years were analysed with monoclonal antibodies for the following markers: inducer-helper, cytotoxic-suppressor, monocytes, Ia, IgG and IgM. Control groups consisted of long-term dialysis patients and a group of normal individuals. The results show a strikingly significant difference between the long-term allograft recipients and the control population in terms of the inversion of the normal inducer-helper to cytotoxic-suppressor ratio. Additionally, there were significant differences in the number of Ia+ cells in long-term transplant patients and dialysis patients compared with normals. No impressive differences were found in the numbers of Ig bearing cells. Thus the long-term allograft state is a clear example of an overabundance of cellular suppression and may explain many of the general phenomena seen in surviving recipients.