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Related Experiment Videos

Rac-E. coli K12 strains carry a preferential attachment site for lambda rev.

R Diaz, K Kaiser

    Molecular & General Genetics : MGG
    |January 1, 1981
    PubMed
    Summary

    Lambda rev is a hybrid phage resulting from recombination between lambda and the Rac prophage in E. coli K12. This study demonstrates that Rac prophage loss occurs via excisive recombination, creating a specific attachment site for lambda rev integration.

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    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Lambda rev is a hybrid lambdoid phage.
    • It arises from recombination between lambda phage and the Rac prophage.
    • The Rac prophage is present in most E. coli K12 strains.

    Purpose of the Study:

    • To investigate the mechanism of Rac prophage loss in E. coli K12.
    • To characterize the integration mechanism of the lambda rev phage.

    Main Methods:

    • Analysis of Rac-E. coli K12 strains.
    • Investigation of excisive recombination events.
    • Characterization of lambda rev integration using its modified int and attP genes.

    Main Results:

    • Three independent Rac-E. coli K12 strains showed specific deletion of the entire Rac prophage.
    • Rac prophage loss occurred through excisive recombination at hybrid attachment sites.
    • Lambda rev integrates site-specifically and in the correct orientation at the attachment site generated by Rac excision.

    Conclusions:

    • Rac prophage excision is a specific genetic event in E. coli K12.
    • Lambda rev utilizes a novel integration strategy involving Rac-derived genetic elements.
    • This recombination mechanism provides a new tool for genetic manipulation in E. coli.

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