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Quantitative model for gene regulation by lambda phage repressor.

G K Ackers, A D Johnson, M A Shea

    Proceedings of the National Academy of Sciences of the United States of America
    |February 1, 1982
    PubMed
    Summary
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    A new model explains how bacteriophage lambda repressor proteins bind cooperatively to DNA operator sites. This interaction is crucial for regulating gene expression and maintaining the lysogenic state.

    Area of Science:

    • Molecular Biology
    • Biophysics
    • Genetics

    Background:

    • Bacteriophage lambda repressor controls gene expression by binding to operator sites.
    • Cooperative binding interactions are essential for precise genetic regulation.

    Purpose of the Study:

    • To develop a statistical thermodynamic model for bacteriophage lambda repressor-operator interactions.
    • To quantitatively interpret cooperative site-specific equilibrium binding data.

    Main Methods:

    • Developed a statistical thermodynamic model.
    • Evaluated interaction parameters using DNase protection experiments in vitro.
    • Predicted repression curves at promoters PR and PRM.

    Main Results:

    Related Experiment Videos

  • Model parameters were determined at 37°C, 0.2 M KCl.
  • Repression at promoter PR is predicted to be nearly complete at physiological repressor concentrations.
  • Promoter PRM is predicted to remain highly active.

    • Conclusions:

    • Cooperative interactions between repressor dimers at OR1 and OR2 are vital for a stable lysogenic state.
    • These interactions facilitate efficient switching to the lytic state during induction.
    • The model provides a quantitative framework for understanding cooperative DNA binding.