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Immune response of rabbits immunized with proteolytic enzymes of B. subtilis
This investigation was carried out to determine antigenic potency of the proteolytic enzymes: Alcalase, Maxatase and crystalline subtilisin type VII. To proof the antigenicity of these enzymes the precipitating antibodies in serum of immunized rabbits were determined. The titre of precipitating antibodies depended on the mode of immunization, preparation and concentration of antigens. The authors compare the effect of the immunization by subcutaneous and intraperitoneal injections of proteolytic enzymes on the production of serum precipitins in rabbits.
This investigation was carried out to determine antigenic potency of the proteolytic enzymes: Alcalase, Maxatase and crystalline subtilisin type VII. To proof the antigenicity of these enzymes the precipitating antibodies in serum of immunized rabbits were determined. The titre of precipitating antibodies depended on the mode of immunization, preparation and concentration of antigens. The authors compare the effect of the immunization by subcutaneous and intraperitoneal injections of proteolytic enzymes on the production of serum precipitins in rabbits.