The continuous measurement of tubular volume changes in response to step changes in contraluminal osmolality

A new method to measure time dependent (t) volume (V) changes in proximal straight tubules (PST) is described. V is calculated from diameter (d) measurements for which a video camera and an integrating circuit are used. A tubular image of high optical contrast is recorded with the TV camera such that the scan lines run crosswise to the tubule. The video signal is analyzed by a special processor which adds 225 tubular diameters of each TV frame and feeds this analog signal to a pen recorder. The fractional error in d measurements is 10(-3). Diameter changes of less than 0.05 micron can be detected, as compared to the usual error of a single measurement of about 0.4 micron. Pcbos, the osmotic water permeability of the contraluminal cell membrane was measured by setting up osmotic steps across it in less than 0.1 s and following the ensuing delta d/delta t. The time delay between solution change and the linear part of the osmotic response was 0.51 +/- 0.05 s. Pcbos was found to be 50.4 (+/- 8.7) X 10(-4) cm3.cm-2 of basement membrane area .s-1.osmolar-1.