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[Action of lincomycin, chymotrypsin and their combinations on the course of experimental staphylococcal infection]
Abstract:
The culture of Staphylococcus aureus was administered intraperitoneally in a dose of LD30 to albino mice. The animals of the 1st, 2nd and 3rd groups were treated with lincomycin, chymotripsin and combination of lincomycin with chymotripsin respectively. The animals of the 4th group were used as control and were not subjected to the treatment with the drugs. A part of the animals from every group was killed on the 3rd, 7th, 14th, 21st and subsequent days and their organs were investigated microscopically and bacteriologically. It was found that staphylococci was isolated from the control mice during a 50-day period after inoculation. Complete liberation of the organs from the causative agent within 25 days from the beginning of the experiment was registered in the animals treated with lincomycin. Isolation of the staphylococci was over by the 27th day in the animals treated with chymotrypsin. Liberation of the organs from the causative agent by the 17th day was observed in the albino mice treated with the combination of lincomycin with chymotrypsin. The combined use of lincomycin with chymotrypsin proved to be most effective: no death was registered among the albino mice, the levels of the pathogenicity and antibiotic resistance in the pathogenic staphylococci decreased.
Insights
The combination of lincomycin and chymotrypsin effectively cleared Staphylococcus aureus infections in mice within 17 days, reducing mortality and antibiotic resistance.
Area of Science:
- Microbiology
- Pharmacology
- Immunology
Context:
- Staphylococcus aureus infections pose significant health risks.
- Antibiotic resistance in S. aureus is a growing concern.
- Evaluating novel therapeutic strategies is crucial.
Purpose:
- To assess the efficacy of lincomycin and chymotrypsin, alone and in combination, against Staphylococcus aureus infection in a murine model.
- To determine the impact of these treatments on bacterial clearance, mortality, and antibiotic resistance.
Summary:
- Albino mice were infected with Staphylococcus aureus (LD30) and treated with lincomycin, chymotrypsin, or a combination.
- Bacteriological and microscopic analyses of organs were performed.
- The combination therapy resulted in complete bacterial clearance by day 17, significantly faster than individual treatments (lincomycin: 25 days, chymotrypsin: 27 days).
- Control mice showed persistent infection for 50 days.
Impact:
- Combined lincomycin and chymotrypsin demonstrated superior efficacy in eradicating S. aureus.
- This combination therapy significantly reduced mortality and decreased the pathogenicity and antibiotic resistance of S. aureus.
- The findings suggest a promising synergistic therapeutic approach for staphylococcal infections.