Immunization with killed Acanthamoeba culbertsoni antigen and amoeba culture supernatant antigen in experimental Acanthamoeba meningoencephalitis

Mice immunized with killed (sonicated) Acanthamoeba culbertsoni A-1 antigen displayed marked resistance to intranasal challenge with the amoeba. A primary immunization produced a survival rate of approximately 40%, and survival values of greater than 80% were obtained by multiple immunizations. Similarly mice immunized with fluids from A-1 cultures were highly resistant to infection.