Histopathological study of the cochlea with altered perilymph metabolism

In 41 guinea pigs an experiment was conducted to determine the effect of kanamycin on ears with or without blockage of the cochlear aqueduct. In 24 animals the cochlear aqueduct was blocked on one side, and after 1 to 21 days, kanamycin (300 mg/kg) was given daily and subcutaneously until the pinna reflex began to decrease. The remaining animals were used for controls: exposure of and trauma to the aperture of the cochlear aqueduct and kanamycin administration (8), blockage of the cochlear aqueduct only (3 plus 17 previous cases), and kanamycin administration to normal animals (6). The survival time of all these animals was four months. The results showed that ears with blockage of the cochlear aqueduct and kanamycin administration consistently demonstrated a more severe and extensive atrophy of the cochlear sensory cells. The lesions occurred primarily at the basal end and spread toward the apical turn, although the lesions in some specimens jumped from the basal to apical turn. The outer hair cells were more extensively affected than the inner hair cells. Proteinaceous precipitates were often found to be slightly greater in the perilymph of cochleae with blocked cochlear aqueducts. From these observations, it is concluded that the decrease in patency of the cochlear aqueduct will impair perilymph metabolism, causing the cochlear sensory cells at the basal turn to become more vulnerable to noxious substances.