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Evaluation of the ASTRA o-cresolphthalein complexone calcium method
American Journal of Clinical Pathology
|August 1, 1984
Summary
This study evaluates an automated calcium assay using o-cresolphthalein complexone chemistry on Beckman ASTRA systems. The method demonstrates excellent accuracy, precision, and minimal interference, making it suitable for clinical use.
Area of Science:
- Clinical Chemistry
- Analytical Chemistry
Background:
- Automated methods are crucial for efficient clinical laboratory diagnostics.
- Accurate calcium measurement is vital for diagnosing various medical conditions.
Purpose of the Study:
- To evaluate the analytical performance of a new automated calcium method.
- To assess its suitability for routine clinical laboratory use.
Main Methods:
- The automated calcium method utilizes o-cresolphthalein complexone chemistry.
- Evaluation involved assessing reproducibility, linearity, recovery, and interferences.
- Comparison with atomic absorption spectrophotometry was performed.
Main Results:
- Day-to-day coefficients of variation ranged from 1.2% to 3.1%.
- Recovery averaged 98% with linearity from 5.0-15.5 mg/dL.
- Positive interference was noted with magnesium; other common substances showed no significant interference.
Conclusions:
- The automated calcium method exhibits acceptable analytical performance and clinical usefulness.
- It offers high throughput (70 samples/hour) and rapid stat results (9 minutes).
- Excellent correlation with atomic absorption spectrophotometry supports its reliability.