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Inherited differences in mouse kidney carnosinase activity.

F L Margolis, M Grillo

    Biochemical Genetics
    |June 1, 1984
    PubMed
    Summary

    Kidney carnosinase activity, an enzyme that breaks down carnosine, shows over 50-fold variation between mouse strains. This variation is inherited as a classical Mendelian trait.

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    Area of Science:

    • Biochemistry
    • Genetics
    • Enzymology

    Background:

    • Carnosinase is a key peptidase responsible for cleaving beta-alanyl-L-histidine (carnosine) and related dipeptides.
    • Significant variability in carnosinase activity has been observed in kidney cytosol across different mouse strains.

    Purpose of the Study:

    • To quantify and characterize interstrain differences in mouse kidney carnosinase activity.
    • To investigate the genetic basis of observed variations in carnosinase levels.

    Main Methods:

    • Assay of carnosinase activity in kidney cytosol from various mouse strains.
    • Crossbreeding experiments (matings and backcrosses) between high- and low-activity strains (NZB/BINJ and BALB/cJ).
    • Analysis of carnosinase activity levels in progeny to determine inheritance patterns.

    Main Results:

    • Mouse kidney carnosinase activity exhibits over 50-fold variation between strains, with high levels in CD-1 and NZB/BINJ mice, and low levels in BALB/cJ and C57BL/6J mice.
    • Carnosinase from high-activity strains is immunologically and enzymologically identical.
    • Progeny from crosses between NZB/BINJ and BALB/cJ strains display carnosinase activity levels consistent with a classical Mendelian trait.

    Conclusions:

    • Significant, genetically determined interstrain differences in mouse kidney carnosinase activity exist.
    • The observed strain distribution and inheritance pattern suggest a single-gene or simple Mendelian control of carnosinase activity.
    • This finding represents the first report of quantitative interstrain differences in carnosinase activity with a defined genetic basis.

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