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Evaluation of automatic antimicrobial susceptibility testing with the MS-2 system.
Bollettino Dell'Istituto Sieroterapico Milanese
|May 31, 1984
Summary
Comparing antibiotic sensitivity testing methods, the Kirby-Bauer agar diffusion method showed better sensitivity for Gram-negative bacteria than the automated MS2 Abbott system. Results were largely consistent, especially for aminoglycosides.
Area of Science:
- Clinical Microbiology
- Infectious Diseases
- Pharmacology
Background:
- Accurate antibiotic sensitivity testing is crucial for effective treatment of Gram-negative bacterial infections.
- Evaluating the performance of different antimicrobial susceptibility testing (AST) methods is essential for clinical decision-making.
Purpose of the Study:
- To compare the sensitivity of the Kirby-Bauer agar diffusion method with the automated MS2 Abbott system for 191 Gram-negative bacteria.
- To assess the concordance rates for various antibiotic classes, including aminoglycosides and cephalosporins.
Main Methods:
- Agar diffusion method (Kirby-Bauer) was employed to test bacterial sensitivity.
- Automated system MS2 Abbott was used as a comparative method.
- Sensitivity and essential agreement percentages were calculated for each antibiotic tested.
Main Results:
- High essential agreement was observed for most antibiotics, with rates ranging from 89% (piperacillin) to 96% (amikacin).
- Aminoglycosides showed no significant differences between the two methods.
- Cephalosporins exhibited a slightly higher incidence of discordance compared to aminoglycosides.
Conclusions:
- The Kirby-Bauer method demonstrated superior sensitivity compared to the automated MS2 Abbott system.
- Both methods provide valuable data, but the Kirby-Bauer method may offer a therapeutic advantage due to its sensitivity.
- The automated system provides rapid Minimum Inhibitory Concentration (MIC) values, aiding in timely antibiotic dose adjustments.