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Related Experiment Videos

Two methods compared for measuring lipase activity in plasma after heparin administration.

C Ehnholm, E A Nikkilä, P Nilsson-Ehle

    Clinical Chemistry
    |September 1, 1984
    PubMed
    Summary

    Two methods for measuring lipase activity in human plasma show good correlation for hepatic lipase (r=0.99) and lipoprotein lipase (r=0.92). Differences in emulsifiers explain variations in lipoprotein lipase measurements.

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    Biochemical Society transactions·2006

    Area of Science:

    • Biochemistry
    • Clinical Chemistry

    Background:

    • Accurate measurement of lipase activity in human plasma is crucial for understanding lipid metabolism.
    • Hepatic lipase (HL) and lipoprotein lipase (LPL) play distinct roles in triglyceride hydrolysis and lipoprotein remodeling.

    Purpose of the Study:

    • To compare two direct, selective methods for measuring HL and LPL activities in human plasma post-heparin administration.
    • To identify factors contributing to discrepancies in LPL activity measurements between the two methods.

    Main Methods:

    • Comparison of two assay protocols for HL and LPL activity using human plasma.
    • Assays differed in emulsifiers (gum arabic vs. lecithin) and LPL activity elimination strategies (immunological inhibition vs. specific substrate).

    Main Results:

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    • High correlation (r=0.99) observed for HL activity measurements between the two methods.
    • LPL assays showed a systematic difference, with one method yielding ~3x higher activity.
    • This difference was attributed to varying emulsifiers, confirmed using purified enzymes.
    • A satisfactory correlation (r=0.92) was found for LPL activity, indicating both methods measure the same enzyme.

    Conclusions:

    • Both methods provide reliable measurements of HL activity.
    • While LPL activity measurements differ systematically due to emulsifiers, both assays are valid for assessing LPL activity.
    • Method selection may depend on specific research or clinical needs, considering the observed differences.