Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

IgE-specific suppressor factors in normal human serum.

D Y Leung, C Brozek, R Frankel

Clinical Immunology and Immunopathology

|September 1, 1984

Summary

Normal human serum contains IgE-binding factors that suppress IgE production. These factors are low molecular weight, isotype-specific, and found in nonallergic individuals, offering potential therapeutic insights.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Quantifying the effects of neutron fluence on proton signal retention in CR-39.

The Review of scientific instruments·2024

Same author

Consensus guidelines for interventional cardiology services delivery during covid-19 pandemic in Australia and new Zealand.

Heart, lung & circulation·2020

Same author

Parental perspectives on decision-making about hypospadias surgery.

Journal of pediatric urology·2019

Same author

Community engagement of adolescents in the development of a patient-centered outcome tool for adolescents with a history of hypospadias repair.

Journal of pediatric urology·2019

Same author

Altered composition of epidermal lipids correlates with Staphylococcus aureus colonization status in atopic dermatitis.

The British journal of dermatology·2017

Same author

Mechanism of male sterility in Petunia: The relationship between pH, callase activity in the anthers, and the breakdown of the microsporogenesis.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2014

Area of Science:

Immunology
Molecular Biology

Background:

Elevated immunoglobulin E (IgE) levels are associated with allergic diseases.
The presence and nature of IgE-specific suppressor factors in human circulation remain unclear.

Purpose of the Study:

To investigate the presence of IgE-specific suppressor factors in the serum of nonallergic individuals.
To characterize the physicochemical properties of these potential suppressor factors.

Main Methods:

Serum from nonatopic donors was filtered to remove IgE.
The IgE-free filtrates were tested for their ability to suppress in vitro IgE synthesis by peripheral blood mononuclear cells (PBMC) from hyper-IgE patients.
Suppressor activity was assessed after enzymatic treatment (trypsin, neuraminidase), heat exposure, and affinity chromatography (IgE-Sepharose, IgG-Sepharose, peanut agglutinin-Sepharose, lentil lectin-Sepharose).

Main Results:

Serum filtrates from all tested nonatopic donors suppressed IgE synthesis by 70% ± 4%.
Suppression was isotype-specific, not affecting IgG or IgM production.
The suppressor activity was heat-labile, trypsin-sensitive, and bound to IgE-Sepharose, indicating IgE-binding capacity.
Factors showed affinity for peanut agglutinin-Sepharose but not lentil lectin-Sepharose.

Conclusions:

Human serum from nonallergic individuals contains low molecular weight IgE-binding factors that selectively suppress IgE production.
These factors share physicochemical similarities with previously described rat T-cell-derived IgE-binding factors.
Further characterization could lead to novel therapeutic strategies for IgE-mediated disorders.

Related Experiment Videos