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[Convenient methods for marking newborn rats].

T Yamada, T Nishiyama, T Inoue

Jikken Dobutsu. Experimental Animals

|April 1, 1984

Summary

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Newborn rat marking using tattooing or branding is safe and effective for up to 21 days. These methods did not impact rat growth, development, or behavior compared to unmarked controls.

Area of Science:

Animal Science
Veterinary Medicine
Laboratory Animal Science

Context:

Accurate identification of individual animals is crucial in research.
Traditional marking methods can sometimes cause stress or affect animal welfare.
Developing reliable and non-invasive marking techniques for neonatal rodents is essential.

Purpose:

To evaluate the efficacy and safety of tattooing and branding as marking methods for newborn rats.
To assess the impact of these marking techniques on animal growth, development, and behavior.
To determine the duration of mark recognizability in young rats.

Summary:

Newborn rats were marked via tattooing (<2x2 mm) or branding (3-4 mm diameter) within 24 hours of birth, excluding cervical and dorsal areas.

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Both marking methods were clearly recognizable for 21 days post-application with no observed mortality.

Marked rats showed no significant differences in body weight gain, external differentiation, or behavior (inclined plane test) compared to controls, even when cared for by foster mothers.

Impact:

Tattooing and branding are viable, safe marking techniques for newborn rats in research settings.
These methods ensure reliable individual identification without compromising animal welfare or data integrity.
The study provides evidence supporting the use of these techniques in neonatal rodent studies.