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Indoleamine-accumulating horizontal cells in the squirrel monkey retina.

I Florén, A Hendrickson

    Investigative Ophthalmology & Visual Science
    |September 1, 1984
    PubMed
    Summary

    Squirrel monkey retinas contain horizontal cells that accumulate serotonin (5HT). These cells, crucial for visual processing, may use a nonfluorescent indole transmitter, not 5HT itself.

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    Area of Science:

    • Neuroscience
    • Ophthalmology
    • Cell Biology

    Background:

    • Horizontal cells play a key role in retinal signal processing.
    • Indoleamines, like serotonin, are neurotransmitters with diverse functions in the nervous system.
    • Understanding neurotransmitter uptake in retinal cells is vital for comprehending visual pathways.

    Purpose of the Study:

    • To investigate the presence and characteristics of indoleamine-accumulating horizontal cells in the Saimiri sciureus retina.
    • To determine if serotonin (5HT) is taken up by these horizontal cells and to localize this uptake.
    • To explore the potential native transmitter of these identified horizontal cells.

    Main Methods:

    • Utilized in vivo and in vitro autoradiography with 3H-serotonin (5HT) for light and electron microscopy.
    • Performed histofluorescence microscopy to visualize indoleamine-accumulating cells.
    • Assessed the effects of light-dark adaptation, temperature, and excess nonradioactive 5HT on uptake.

    Main Results:

    • Identified horizontal cells in the squirrel monkey retina capable of selectively accumulating 3H-5HT.
    • Localized 5HT uptake to both the somas and processes within the synaptic invaginations of cone and rod photoreceptors.
    • Demonstrated temperature dependence and saturability of 5HT uptake, suggesting a specific transport mechanism.
    • Observed that these cells lack intrinsic fluorescence, indicating 5HT is not their native transmitter.

    Conclusions:

    • Squirrel monkey horizontal cells possess a specific mechanism for accumulating indoleamines, including serotonin.
    • Serotonin is unlikely to be the native neurotransmitter of these horizontal cells due to their lack of intrinsic fluorescence.
    • The native transmitter is hypothesized to be a related, nonfluorescent indole compound.

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