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Updated: Aug 19, 2026

Preparation of Living Isolated Vertebrate Photoreceptor Cells for Fluorescence Imaging
Published on: June 22, 2011
Flash photolysis of rhodopsin in rabbit
Abstract:
Flash photolysis of rhodopsin in rabbit's retina has been analysed theoretically, and the results are found to be in good agreement with the experimental results of Hagins (1957). We have also obtained the variation of relative concentrations of rhodopsin, lumirhodopsin, isorhodopsin and metarhodopsin I during the period of the flash corresponding to two different intensities of the flash. It has been found that the quantum efficiencies of conversion of lumirhodopsin into rhodopsin and isorhodopsin will lie in the range 0.24--0.45 and 0.20--0.44 respectively; quantum efficiency of conversion of metarhodopsin I into rhodopsin and isorhodopsin are found to have values greater than 0.52 and 0.45 respectively and the quantum efficiency of conversion of isorhodopsin into lumirhodopsin has been found to be approximately 0.865. Also the maximum value of the rate constant of the reaction metarhodopsin I leads to metarhodopsin II at 37 degrees C has been determined in decerebrated eye and it has been found that it is of the same order as found by Pugh (1975) in the case of human eye.
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