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Growth of clinical isolates in the cold
Medical Microbiology and Immunology
|January 1, 1984
Abstract:
Common clinical isolates were tested to determine their ability to multiply after 7 days at 4 degrees C in trypticase soy broth. 34% of the 214 isolates grew in the cold. Citrobacter freundii, Group D Enterococci, Klebsiella oxytoca, Proteus vulgaris, Providencia rettgeri and Serratia marcescens grew best of all. The importance of growth in the cold is discussed.
Insights
Many common clinical bacteria can grow in cold temperatures. This study found 34% of tested isolates multiplied at 4°C, with specific species like Citrobacter freundii showing significant cold growth.
Area of Science:
- Microbiology
- Food Safety
- Clinical Diagnostics
Background:
- Cold temperatures are often considered inhibitory to bacterial growth.
- Understanding bacterial proliferation at low temperatures is crucial for food safety and clinical settings.
Purpose of the Study:
- To assess the cold growth potential of common clinical bacterial isolates.
- To identify specific bacterial species that can multiply at refrigeration temperatures (4°C).
Main Methods:
- 214 common clinical isolates were incubated in trypticase soy broth at 4°C for 7 days.
- Bacterial growth was quantified to determine multiplication capacity.
Main Results:
- 34% of the 214 clinical isolates demonstrated growth after 7 days at 4°C.
- Citrobacter freundii, Group D Enterococci, Klebsiella oxytoca, Proteus vulgaris, Providencia rettgeri, and Serratia marcescens exhibited the most robust cold growth.
Conclusions:
- A significant proportion of common clinical bacteria possess the ability to grow at 4°C.
- The findings highlight the importance of considering cold growth in bacterial contamination control and food safety protocols.