[Gas chromatography determination of atropine, theophylline, phenobarbital and aminophenazone in tablets]

A GLC method consisting of two runs has been developed for the quantitative determination of atropine, theophylline, phenobarbital and aminophenazone in tablets. By using simple extraction procedures two fractions are obtained. Atropine is silylated with BSA and homatropine is applied as the internal standard. Theophylline, phenobarbital and aminophenazone are treated with trimethylphenylammonium acetate and amobarbital is used as the internal standard. Separations are carried out on 3% OV 1 at 160 degrees C and 210 degrees C. The method is applicable for determining atropine even when its amount is 0.07% of that of the sample. The procedure presented here provides possibilities for the assay of these compounds in various dosage forms.