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Related Experiment Videos

Intraperitoneal immunization against necrobacillosis in experimental animals.

M M Garcia, K A McKay

    Canadian Journal of Comparative Medicine : Revue Canadienne De Medecine Comparee
    |January 1, 1978
    PubMed
    Summary

    Intraperitoneal immunization with Fusobacterium necrophorum cytoplasm protected mice and sheep against lethal pathogen challenge. This strategy cleared bacteria from livers within 24 hours, preventing abscess formation.

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    Area of Science:

    • Veterinary Immunology
    • Bacteriology
    • Infectious Diseases

    Background:

    • Fusobacterium necrophorum is a significant pathogen causing liver abscesses in livestock.
    • Effective vaccines against F. necrophorum are currently lacking.
    • Intracellular antigens are potential targets for vaccine development.

    Purpose of the Study:

    • To evaluate the efficacy of intraperitoneal immunization with F. necrophorum cytoplasm against a lethal challenge.
    • To determine the optimal immunization schedule for protection.
    • To assess the protective role of sera from immunized animals.

    Main Methods:

    • Mice were immunized intraperitoneally with F. necrophorum cytoplasm and challenged with the pathogen.
    • Sheep were immunized with cytoplasmic protein and challenged.

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  • Liver bacterial loads and abscess development were monitored.
  • Sera transfer experiments were conducted.

    • Main Results:

    • Intraperitoneal immunization with F. necrophorum cytoplasm conferred complete protection in mice against a lethal challenge.
    • A schedule of six weekly intraperitoneal doses was critical for complete protection in mice.
    • Livers of immunized mice were cleared of bacteria within 24 hours post-challenge.
    • Sheep immunized with cytoplasmic protein were protected against abscess development.
    • Sera from immunized animals did not protect recipient mice from liver abscesses.

    Conclusions:

    • Intraperitoneal immunization with F. necrophorum cytoplasm is a promising strategy for preventing F. necrophorum-induced liver abscesses.
    • The intracellular fraction of F. necrophorum is a key target antigen for vaccine development.
    • Further research into the mechanisms of protection and potential for broader application is warranted.