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Related Experiment Videos

Three-dimensional structure of bladder membrane protein.

R H Wade, A Brisson

    Ultramicroscopy
    |January 1, 1984
    PubMed
    Summary
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    Crystals in mammalian bladder membranes exhibit a p6 plane group structure. Three-dimensional reconstruction reveals a twelve-subunit motif, with protein particles located on one side of the membrane.

    Area of Science:

    • Biophysics
    • Structural Biology
    • Cell Biology

    Background:

    • Mammalian bladder membranes possess naturally occurring crystalline structures.
    • Understanding the structural organization of these membranes is crucial for comprehending their function.

    Purpose of the Study:

    • To determine the crystallographic structure of mammalian bladder membrane crystals.
    • To elucidate the arrangement and distribution of protein subunits within the membrane.

    Main Methods:

    • Crystallographic analysis of naturally occurring mammalian bladder membrane crystals.
    • Three-dimensional (3D) reconstruction using negative stain electron microscopy.
    • Comparison with shadowed specimens to identify protein particle location.

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    Main Results:

    • The crystals belong to the two-sided plane group p6 with a lattice parameter of 160 Å.
    • 3D reconstruction revealed a motif of twelve subunits, elongated perpendicular to the crystal plane to a height of approximately 50 Å.
    • Negative stain revealed protein particles exclusively on one side of the membrane, confirmed by comparison with shadowed specimens.

    Conclusions:

    • The mammalian bladder membrane exhibits a well-defined crystalline structure.
    • The protein subunits are asymmetrically distributed, primarily on one surface of the membrane.
    • These findings provide insights into the structural basis of bladder membrane organization and function.