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Yersinia spp. in surface water in Matsue, Japan
Abstract:
Yersinia spp. (741 strains) were recovered from 81% of 48 surface water samples collected over a 12-month period from four rivers in Matsue, Japan. The precipitation methods with FeCl3 or Kaolin and the cold enrichment method with Peptone-Mannitol-Phosphate buffer solution were used for recovery. Isolates belonged to Yersinia enterocolitica (Ye) (133 strains), Yersinia intermedia (511 strains), Yersinia frederiksenii (57 strains), Yersinia kristensenii (10 strains) and X2-like organisms (30 strains). Thirty colonies of Ye O3 biotype 3 per ml surface water may relate to the drainage containing 2 X 10(4) Ye O3 biotype 3 per ml, from the piggery that raised Ye O3 biotype 3-positive pigs. There was the negative interrelation between the incidence of isolation of Yersinia spp. and the environmental- and water temperatures. This may be the first documentation of isolation of Ye O3 from surface water.
Insights
Yersinia species were found in 81% of Japanese river water samples. Yersinia enterocolitica O3 was detected, potentially linked to piggery drainage, marking its first surface water isolation.
Area of Science:
- Environmental microbiology
- Water quality monitoring
- Bacteriology
Background:
- Yersinia species are significant human and animal pathogens.
- Surface water can act as a reservoir for Yersinia.
- Understanding Yersinia distribution in aquatic environments is crucial for public health.
Purpose of the Study:
- To investigate the prevalence and diversity of Yersinia species in Japanese surface waters.
- To identify potential sources and environmental factors influencing Yersinia isolation.
- To document the first isolation of Yersinia enterocolitica O3 from surface water.
Main Methods:
- Collection of 48 surface water samples over 12 months from four rivers in Matsue, Japan.
- Utilized precipitation methods (FeCl3 or Kaolin) and cold enrichment (Peptone-Mannitol-Phosphate buffer) for Yersinia recovery.
- Species identification included Yersinia enterocolitica, Yersinia intermedia, Yersinia frederiksenii, Yersinia kristensenii, and X2-like organisms.
Main Results:
- Yersinia spp. were recovered from 81% of samples, with 741 strains identified.
- Yersinia intermedia was the most prevalent species (511 strains), followed by Yersinia enterocolitica (133 strains).
- Yersinia enterocolitica O3 biotype 3 was detected in surface water, with potential links to piggery drainage.
Conclusions:
- Japanese rivers harbor diverse Yersinia species, with Yersinia intermedia being dominant.
- Environmental and water temperatures showed a negative correlation with Yersinia spp. isolation.
- The study reports the first isolation of Yersinia enterocolitica O3 from surface water, highlighting potential risks.