Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Effect of heparin on platelet aggregation.

H I Saba, S R Saba, G A Morelli

    American Journal of Hematology
    |October 1, 1984
    PubMed
    Summary

    Heparin potentiates platelet aggregation in plasma but inhibits it in isolated platelets. This antiaggregatory effect, specific to heparin, is neutralized by heparinase and protamine, suggesting complex interactions with platelet function.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    IgM Myeloma: An IgM Gammopathy Distinct from Waldenstrom's Macroglobulinemia.

    Hematology (Amsterdam, Netherlands)·2016
    Same author

    Estimated Blood Loss in Group O as Compared to Non-group O Radical Prostatectomy Patients.

    Hematology (Amsterdam, Netherlands)·2016
    Same author

    Efficacy of decitabine in the treatment of patients with chronic myelomonocytic leukemia (CMML).

    Leukemia research·2007
    Same author

    Cardiac natriuretic peptides: hormones with anticancer effects that localize to nucleus, cytoplasm, endothelium, and fibroblasts of human cancers.

    Histology and histopathology·2006
    Same author

    Efficacy of NovoSeven during surgery on a haemophiliac with previous history of inhibitors.

    Haemophilia : the official journal of the World Federation of Hemophilia·2003
    Same author

    IL-1 and TNF independent pathways mediate ICAM-1/VCAM-1 up-regulation in ischemia reperfusion injury.

    Journal of leukocyte biology·2001

    Area of Science:

    • Biochemistry
    • Hematology
    • Pharmacology

    Background:

    • Heparin is widely used as an anticoagulant.
    • Its effects on platelet aggregation are complex and context-dependent.

    Purpose of the Study:

    • To systematically investigate the effect of heparin on platelet aggregation in various platelet preparations.
    • To elucidate the mechanisms underlying heparin's influence on platelet function.

    Main Methods:

    • Examined heparin's effect on platelets in platelet-rich plasma (PRP), gel-filtered, washed, and formaldehyde-fixed platelets.
    • Tested various agonists including ADP, arachidonic acid, thrombin, and ristocetin.
    • Assessed thromboxane generation and response to preformed thromboxane.

    Main Results:

    • Heparin showed mild potentiation in PRP but significant inhibition in isolated platelets.
    • Inhibitory activity was specific, neutralized by heparinase and protamine.
    • Most pronounced inhibition occurred with ristocetin.
    • Heparin enhanced thromboxane generation but impaired platelet response to agonists.

    Conclusions:

    • Heparin exhibits context-dependent effects on platelet aggregation.
    • It may alter platelet membrane function, interfering with agonist response.
    • Plasma factors inhibit heparin's antiaggregatory activity.

    Related Experiment Videos