Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rat-colonic, mucus glycoprotein.

V L Murty, F J Downs, W Pigman

    Carbohydrate Research
    |March 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Researchers isolated a homogeneous glycoprotein from rat colonic mucosa. This complex molecule, rich in carbohydrates and specific amino acids, features unique O-glycosidic linkages and branched side chains.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    British Families Education Service in Germany.

    Nature·2010
    Same author

    Salivary mucins in oral mucosal defense.

    General pharmacology·1996
    Same author

    Inhibition of gastric mucosal mucin receptor by H. pylori lipopolysaccharide: effect of ebrotidine.

    General pharmacology·1995
    Same author

    Gastric mucus viscosity and Helicobacter pylori.

    Gut·1995
    Same author

    Enhancement in gastric mucosal EGF and PDGF receptor expression with ulcer healing by sulglycotide.

    General pharmacology·1995
    Same author

    Susceptibility of Helicobacter pylori to antimicrobial agents: effect of sulglycotide.

    Biochemistry and molecular biology international·1995
    Same journal

    Preparation of fucose-containing galacto-oligosaccharides using a commercial β-galactosidase from Bifidobacterium bifidum.

    Carbohydrate research·2026
    Same journal

    Two distinct sulfated O-polysaccharides from the marine bacterium Vibrio sp. KMM 9700: Genomic prediction and structural elucidation.

    Carbohydrate research·2026
    Same journal

    Binding evaluation of poacic acid for fungal cell-wall glycans and β-1,3-glucooligosaccharides.

    Carbohydrate research·2026
    Same journal

    Chemoenzymatic synthesis of unusual antibody glycoforms using E. coli expressed human and bovine β-1,4-galactosyltransferase and their mutants.

    Carbohydrate research·2026
    Same journal

    Bioactive 13,28-oxidooleanane-type saponins from Measa kamerunensis Merk.

    Carbohydrate research·2026
    Same journal

    Stereoselective synthesis of 1,2-annulated C-glycosides via palladium-catalyzed intramolecular annulation of C-aryl glycosides.

    Carbohydrate research·2026
    See all related articles

    Area of Science:

    • Biochemistry
    • Glycobiology
    • Gastroenterology

    Background:

    • Mucus glycoproteins are crucial for mucosal protection and lubrication.
    • Understanding the structure of colonic glycoproteins aids in elucidating their physiological roles.

    Purpose of the Study:

    • To isolate and characterize a glycoprotein from rat colonic mucosa.
    • To determine its molecular weight, composition, and structural features, including glycosidic linkages and amino acid profile.

    Main Methods:

    • Isolation of glycoprotein from rat colonic mucosa.
    • Analytical ultracentrifugation to determine molecular weight and homogeneity.
    • Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) to assess subunits.
    • Chemical analysis of carbohydrate and amino acid composition.

    Related Experiment Videos

  • Alkaline borohydride cleavage to identify glycosidic linkages.

    • Main Results:

    • A homogeneous glycoprotein with a molecular weight of 9.0 x 10(5) Da was isolated.
    • The glycoprotein comprised 14% protein and 86% carbohydrate, with galactose, fucose, and sialic acid as major sugars.
    • It contained sulfate but no uronic acid, and exhibited high proportions of serine, threonine, Asx, and Glx.
    • O-glycosidic linkages were identified between N-acetylhexosamine and serine/threonine residues.
    • Carbohydrate side chains averaged 22 units in length and were branched.

    Conclusions:

    • The isolated rat colonic glycoprotein shares similarities with other mammalian mucus glycoproteins but possesses unique compositional characteristics.
    • The identified O-glycosidic linkages and branched carbohydrate structures provide insights into the glycoprotein's functional properties.
    • Further research into this glycoprotein could illuminate its role in colonic function and disease.