Morphological observations on frozen and thawed muscovy spermatozoa

Fresh Muscovy drake spermatozoa were examined using a scanning electron microscope (SEM). The average lengths of the segments were: acrosome 1.8 micron, nucleus 10.9 micron, midpiece 3.6 micron and flagellum (exclusive of midpiece) 71 micron. Under the light microscope, the incidence of abnormal spermatozoa in Muscovy semen subjected to freezing and thawing (almost all with crooked necks) was about 5% higher than that in diluted unfrozen semen. In thawed semen, various abnormalities of the acrosome were observed under the SEM. It seemed that the most radical change was the complete separation of the acrosome from the apical part of the nucleus. The incidence of abnormal acrosomes was increased more than 20% by freezing and thawing. These results suggest that low fertility in thawed semen may be related to increases in the proportion of spermatozoa with crooked necks and acrosomal damage.