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Modified radioassay for measuring asialoglycoprotein in serum
Clinical Chemistry
|October 1, 1984
Summary
This study developed an assay to measure asialoglycoproteins (ASAG) in human serum. Elevated ASAG levels indicate liver disease, aiding in diagnosis.
Area of Science:
- Biochemistry
- Hepatology
- Clinical Chemistry
Background:
- Asialoglycoproteins are cleared from circulation by hepatic binding proteins.
- A sensitive assay is needed to detect asialoglycoprotein levels in human serum.
Purpose of the Study:
- To develop and validate an inhibition assay for detecting asialoglycoprotein in human serum.
- To assess the utility of this assay in identifying liver disease.
Main Methods:
- Developed an inhibition assay using 125I-labeled asialo-alpha 1-acid glycoprotein (ASAG) and purified hepatocyte membranes.
- Measured ASAG inhibitor concentrations in serum from healthy controls and patients with various liver conditions.
Main Results:
- The assay reliably detected 1-20 ng of ASAG.
- Mean ASAG inhibitor concentration in healthy controls was 0.153 mg/L.
- Significantly elevated ASAG levels were found in patients with malignant extrahepatic biliary obstruction, alcoholic liver disease, viral hepatitis, and liver metastases.
Conclusions:
- The developed assay is sensitive and reproducible for quantifying ASAG in human serum.
- Increased ASAG levels are associated with several liver diseases, suggesting diagnostic potential.