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Correlative variations of the free energies for enzyme-substrate complex formation and the transition-state
FEBS Letters
|October 1, 1984
Abstract:
It was found for RNases of different specificities that changes in the free energy for substrate-enzyme binding induced by variations in the nucleotide base structure are accompanied by proportional changes in kcat/Km. This was considered to be a consequence of the strain in the enzyme-substrate complex.