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Lymphocyte sub-populations and NSAIDs. Methodological aspects.

P A Bacon, M Salmon, A Blann

    Inflammation
    |June 1, 1984
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    Activated lymphocytes, identified as the less dense lymphocyte population (LDL), are elevated in rheumatoid arthritis (RA) and correlate with active synovitis. A new technique aids in characterizing these cells for better understanding of RA pathogenesis.

    Area of Science:

    • Immunology
    • Rheumatology

    Background:

    • Activated lymphocytes, defined as the less dense lymphocyte population (LDL), are implicated in autoimmune diseases.
    • Elevated LDL levels have been observed in rheumatoid arthritis (RA).

    Purpose of the Study:

    • To develop and validate a cytospin technique for enumerating and characterizing LDL in rheumatoid arthritis.
    • To investigate the correlation of LDL with active synovitis and extra-articular disease in RA.

    Main Methods:

    • Enumeration of less dense lymphocyte population (LDL) using Ficoll density gradient.
    • Characterization of LDL using a novel cytospin technique and monoclonal antibodies.
    • Comparison of LDL levels and characteristics in RA patients with active synovitis, active disease controls, and healthy individuals.

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    Main Results:

    • LDL numbers are higher in RA patients and decrease with effective therapy.
    • LDL levels correlate with active synovitis, not extra-articular disease.
    • The cytospin technique confirms LDL are T-depleted and enriched in IA bearing cells, potentially involved in synovium homing.
    • RA patients with active synovitis and active disease controls showed increased LDL and IA bearing cells.

    Conclusions:

    • The developed cytospin technique provides a sensitive method for LDL characterization in RA.
    • Elevated LDL and IA bearing cells are associated with active synovitis in RA.
    • These findings suggest a role for LDL in the pathogenesis of RA synovitis.