Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A fast method for processing biopsy material for electron microscopy.

D Baic, B Baic

    Ultrastructural Pathology
    |January 1, 1984
    PubMed
    Summary

    A new 4-hour method simplifies electron microscopy sample preparation for biopsies. This rapid fixation and embedding technique uses ready-to-use ampules and chemical dehydration for excellent results.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Axial differences in the musculature of uropeltid snakes: the freight-train approach to burrowing.

    Science (New York, N.Y.)·1978
    Same author

    Regional specialization of reptilian scale surfaces: relation of texture and biologic role.

    Science (New York, N.Y.)·1977
    Same author

    Argon laser micro-irradiation of mitochondria in rat myocardial cells in tissue culture. IV. Ultrastructural and cytochemical analysis of minimal lesions.

    Journal of molecular and cellular cardiology·1973
    Same author

    Intracisternal fibers in the liver cells of starved frogs.

    Journal of ultrastructure research·1973
    Same author

    Cadmium-induced selective lesions of sensory ganglia.

    Journal of neuropathology and experimental neurology·1967
    Same author

    Toxicity of cadmium for the central nervous system.

    Experimental neurology·1967

    Area of Science:

    • Electron microscopy
    • Biopsy processing
    • Pathology

    Background:

    • Electron microscopy requires meticulous sample preparation.
    • Traditional methods for biopsy fixation and embedding are time-consuming.
    • Optimizing sample preparation is crucial for diagnostic accuracy.

    Purpose of the Study:

    • To develop a rapid method for electron microscopic examination of biopsy material.
    • To simplify the fixation and embedding procedures.
    • To reduce the overall processing time for biopsy samples.

    Main Methods:

    • Biopsies were fixed using ready-to-use ampules.
    • Chemical dehydration was performed using 2,2-dimethoxypropane.
    • The entire procedure was streamlined for speed and efficiency.

    Main Results:

    • The developed method significantly reduces sample preparation time.
    • The procedure yields excellent results for electron microscopic examination.
    • The simplified steps enhance ease of use in the laboratory.

    Conclusions:

    • This rapid method offers a viable alternative for electron microscopy of biopsies.
    • The technique provides high-quality results in a significantly shorter timeframe.
    • The 4-hour procedure is suitable for routine diagnostic applications.

    Related Experiment Videos