Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A cold stage for the Philips EM300 electron microscope.

K A Taylor, R A Milligan, C Raeburn

    Ultramicroscopy
    |January 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    MEN2 phenotype in a family with germline heterozygous rare RET K666N variant.

    Endocrinology, diabetes & metabolism case reports·2024
    Same author

    Biomarkers and longitudinal changes in lumbar spine degeneration and low back pain: the Johnston County Osteoarthritis Project.

    Osteoarthritis and cartilage·2023
    Same author

    Equine chlamydiosis-An emerging infectious disease requiring a one health surveillance approach.

    Zoonoses and public health·2017
    Same author

    The contribution of zinc to platelet behaviour during haemostasis and thrombosis.

    Metallomics : integrated biometal science·2016
    Same author

    Amplification of human platelet activation by surface pannexin-1 channels.

    Journal of thrombosis and haemostasis : JTH·2014
    Same author

    The Direct Assessment of Nonvertebral Fractures in Community Experience (DANCE) study: 2-year nonvertebral fragility fracture results.

    Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2013

    A new cold stage for electron microscopes allows detailed investigation of frozen-hydrated biological structures. This cryo-stage achieves high resolution at -120°C with stable specimen maintenance.

    Area of Science:

    • Cryo-electron microscopy
    • Biological specimen preparation
    • Microscopy instrumentation

    Background:

    • Investigating biological structures requires high-resolution imaging techniques.
    • Frozen-hydrated specimens offer native structural preservation.
    • Existing electron microscopy setups may require specialized stages for cryo-specimens.

    Purpose of the Study:

    • To develop and evaluate a novel cold stage for Philips electron microscopes (EM300/EM301).
    • To enable high-resolution imaging of frozen-hydrated biological specimens.
    • To facilitate easy specimen exchange and long-term stability at low temperatures.

    Main Methods:

    • Construction of a minimal-modification cold stage for Philips EM300/EM301.
    • Operation at a standard temperature of -120°C.

    Related Experiment Videos

  • Testing specimen exchange and stability protocols.

    • Main Results:

    • Achieved resolution better than 10 Å at -120°C.
    • Demonstrated successful, rapid specimen exchange without condensation or warming.
    • Maintained specimen integrity for several hours without detectable deterioration.

    Conclusions:

    • The developed cold stage is effective for high-resolution cryo-electron microscopy of biological samples.
    • Minimal instrument modification and ease of use are key advantages.
    • The stage provides a stable environment for prolonged observation of frozen-hydrated specimens.