Jove
Visualize
Contact Us

Related Experiment Videos

Tetracycline diffusion through phospholipid bilayers and binding to phospholipids.

M Argast, C F Beck

    Antimicrobial Agents and Chemotherapy
    |August 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The chloroplastic GrpE homolog of Chlamydomonas: two isoforms generated by differential splicing.

    The Plant cell·2001
    Same author

    Characterization of Chlamydomonas mutants defective in the H subunit of Mg-chelatase.

    Molecular genetics and genomics : MGG·2001
    Same author

    Signaling pathways in chloroplast-to-nucleus communication.

    Protist·2001
    Same author

    Possible role for molecular chaperones in assembly and repair of photosystem II.

    Biochemical Society transactions·2001
    Same author

    Low-expression genes induced by nitrogen starvation and subsequent sexual differentiation in Chlamydomonas reinhardtii, isolated by the differential display technique.

    Planta·2001
    Same author

    The phototropin family of photoreceptors.

    The Plant cell·2001
    JoVE
    x logofacebook logolinkedin logoyoutube logo
    ABOUT JoVE
    OverviewLeadershipBlogJoVE Help Center
    AUTHORS
    Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
    LIBRARIANS
    TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
    RESEARCH
    JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
    EDUCATION
    JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
    Terms & Conditions of Use
    Privacy Policy
    Policies

    Tetracycline can diffuse through lipid bilayers, as shown by liposome studies. The antibiotic also binds to phospholipids, influencing its membrane transport.

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Pharmacology

    Background:

    • Understanding drug transport across cell membranes is crucial for drug delivery and efficacy.
    • Tetracycline is a widely used antibiotic with a known mechanism of action, but its membrane permeability details require further elucidation.

    Purpose of the Study:

    • To investigate the ability of tetracycline to permeate phospholipid bilayers via diffusion.
    • To determine if tetracycline interacts with or binds to lipid bilayers and phospholipids.

    Main Methods:

    • Utilized liposomes as model systems to study drug diffusion and retention.
    • Employed liposomes containing entrapped Tet repressor protein to assess tetracycline accumulation.
    • Performed binding assays with isolated phospholipids.

    Related Experiment Videos

    Main Results:

    • Liposomes did not retain encapsulated tetracycline, indicating passage through the bilayer.
    • Tetracycline accumulation was observed in liposomes containing Tet repressor protein.
    • The antibiotic demonstrated binding to both liposomes and isolated phospholipids.

    Conclusions:

    • Tetracycline can effectively pass through phospholipid bilayers by diffusion.
    • Tetracycline exhibits binding affinity towards phospholipids, which may influence its membrane transport dynamics.