Quantitative affinity chromatography: further developments in the analysis of experimental results from column...

The problem of allowance for the effects of gel partitioning of the solute in quantitative affinity chromatography has been reexamined, and new expressions have been derived for the description of results obtained by frontal chromatography. In addition, the theoretical expressions have been rearranged into forms suitable for determination of the relevant interaction parameters by simple graphical analysis, a procedure illustrated with results obtained in a partition equilibrium study of the NADH-dependent elution of rabbit muscle lactate dehydrogenase from Blue Sepharose.