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Related Experiment Videos

PTH release stimulated by high extracellular potassium is associated with a decrease in cytosolic calcium in bovine

D M Shoback, E M Brown

    Biochemical and Biophysical Research Communications
    |September 17, 1984
    PubMed
    Summary

    High extracellular potassium (K+) stimulates parathyroid hormone (PTH) release by decreasing, not increasing, cytosolic calcium (Ca++) levels. This finding contrasts with most secretory cells, revealing a unique mechanism for PTH secretion.

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    Area of Science:

    • Endocrinology
    • Cell Physiology
    • Molecular Biology

    Background:

    • Parathyroid hormone (PTH) regulates calcium homeostasis.
    • Cytosolic calcium (Ca++) is a key signaling molecule in many cellular processes, including hormone release.
    • The precise mechanisms regulating PTH release, particularly the role of intracellular calcium under varying extracellular potassium conditions, require further elucidation.

    Purpose of the Study:

    • To investigate the role of cytosolic calcium (Ca++) in parathyroid hormone (PTH) release stimulated by high extracellular potassium (K+).
    • To determine if elevated extracellular K+ leads to an increase or decrease in intracellular Ca++ during PTH secretion.

    Main Methods:

    • Utilized the intracellular calcium (Ca++)-sensitive dye QUIN-2 to measure cytosolic Ca++ concentrations.

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  • Exposed cells to varying concentrations of extracellular calcium (Ca++) and potassium (K+).
  • Quantified PTH release under different ionic conditions.

    • Main Results:

    • High extracellular potassium (K+) concentrations significantly decreased cytosolic calcium (Ca++) levels in parathyroid cells.
    • This reduction in cytosolic Ca++ was directly attributable to high K+ and not to changes in osmotic pressure.
    • Stimulation of PTH release by high K+ was observed concurrently with a decrease in cytosolic Ca++, irrespective of extracellular Ca++ levels.

    Conclusions:

    • Contrary to most secretory cells, high extracellular potassium (K+)-induced parathyroid hormone (PTH) release is associated with a decrease in cytosolic calcium (Ca++).
    • This study reveals a distinct signaling pathway for PTH secretion, highlighting the complex regulation of calcium homeostasis.