Clonidine-induced feeding: analysis of central sites of action and fiber projections mediating this response

Clonidine (CLON), an alpha-adrenergic agonist, was used in conjunction with norepinephrine (NE) to elicit feeding in satiated rats that had sustained hypothalamic electrolytic lesions, or coronal knife cuts at the hypothalamic, midbrain or pontine level of the brainstem. Electrolytic lesions of the paraventricular nucleus (PVN) of the hypothalamus significantly attenuated feeding normally stimulated by intraperitoneal injection of CLON. This contrasts with lesions in the dorsomedial or perifornical hypothalamic regions which had no effect on CLON-elicited eating. Knife cuts placed in the posterior hypothalamus and throughout the midbrain tegmentum also left intact the CLON eating response, in contrast to specific cuts in the dorsal pontine tegmentum which disrupted feeding elicited by PVN injections of NE and CLON, as well as by peripheral administration of CLON. Analyzed together, these results with effective and ineffective cuts relative to CLON and NE feeding provide evidence for an alpha-adrenergic feeding circuit which originates in the PVN and descends from this nucleus, via a dorsal periventricular course, through the diencephalon and midbrain. Further caudally, these fibers mediating NE and CLON feeding then appear to traverse ventrolaterally into the dorsolateral pontine tegmentum on their way to the dorsal medulla.