Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Levels of histone H4 diacetylation decrease dramatically during sea urchin embryonic development and correlate with

S A Chambers, B R Shaw

    The Journal of Biological Chemistry
    |November 10, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Charge Transfer and Built-in Electric Fields between a Crystalline Oxide and Silicon.

    Physical review letters·2019
    Same author

    Electronic and magnetic properties of epitaxial perovskite SrCrO₃(0 0 1).

    Journal of physics. Condensed matter : an Institute of Physics journal·2015
    Same author

    Reversible nano-structuring of SrCrO3-δ through oxidation and reduction at low temperature.

    Nature communications·2014
    Same author

    Impact of lattice mismatch and stoichiometry on the structure and bandgap of (Fe,Cr)2O3 epitaxial thin films.

    Journal of physics. Condensed matter : an Institute of Physics journal·2014
    Same author

    Band alignment, built-in potential, and the absence of conductivity at the LaCrO3/SrTiO3(001) heterojunction.

    Physical review letters·2011
    Same author

    Attitudinal and demographic determinants of diet quality and implications for policy targeting.

    Journal of human nutrition and dietetics : the official journal of the British Dietetic Association·2011
    Same journal

    Isotope-Edited ESEEM: A New Method for Probing Copper Binding Sites in Neurodegenerative Proteins.

    The Journal of biological chemistry·2026
    Same journal

    Introduction to the Thematic Review Series on Intracellular Protein Degradation. The ubiquitous biology of intracellular protein degradation: a tribute to Alfred L. ("Fred") Goldberg.

    The Journal of biological chemistry·2026
    Same journal

    Correction: Aromatic residue-rich amino-terminal segments of temporin L self-assemble into collagen-mimetic peptides with cell-adhesion properties.

    The Journal of biological chemistry·2026
    Same journal

    YhbO is a DJ-1 family glyoxalase and α-oxoaldehyde hydratase that confers resistance to reactive carbonyl stress (112).

    The Journal of biological chemistry·2026
    Same journal

    ARMH3 acts as a central scaffold at the Golgi/TGN through interactions with Arl5, GBF1, and PI4KB.

    The Journal of biological chemistry·2026
    Same journal

    PAX8 controls proximal tubule epithelial identity and stress response through epigenetic modification of distal regulatory elements.

    The Journal of biological chemistry·2026
    See all related articles

    Histone H4 acetylation levels decrease significantly after sea urchin blastulation. This suggests diacetylated histone H4 plays a crucial role in early embryonic chromatin maturation and cell division.

    Area of Science:

    • Developmental Biology
    • Molecular Biology
    • Chromatin Biology

    Background:

    • Histone modifications, such as acetylation, are critical for regulating gene expression and chromatin structure.
    • The dynamic changes in histone acetylation during early development are not fully understood.
    • Histone H4 acetylation patterns vary across different developmental stages in sea urchins.

    Purpose of the Study:

    • To investigate the role of histone H4 acetylation in early sea urchin development.
    • To analyze the changes in histone H4 acetylation during different embryonic stages.
    • To correlate histone H4 acetylation levels with cell division rates.

    Main Methods:

    • In situ protamine release of chromosomal proteins.
    • Triton/acid/urea-polyacrylamide gel electrophoresis.

    Related Experiment Videos

  • In vivo labeling with [3H]acetic acid and [3H]lysine.

    • Main Results:

    • Diacetylated histone H4 (H4aa) is the predominant form in early sea urchin embryos (2-cell to 64-cell stages).
    • Unmodified histone H4 (H4O) becomes predominant after blastulation.
    • H4aa levels are significantly higher in early, rapidly dividing embryos compared to later stages.
    • Enhanced histone H4 acetylation correlates with cell doubling rates and decreases as nucleosomal repeat length increases.

    Conclusions:

    • Chromatin structure dynamically changes throughout sea urchin development.
    • Diacetylated histone H4 plays an intimate role in the maturation of newly replicated chromatin.
    • Histone H4 acetylation levels are closely linked to the rate of cell proliferation during early embryogenesis.