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A cell-cycle-phase-specific mutant of amoeba.

A Gangopadhyay, S Chatterjee

    Journal of Cell Science
    |June 1, 1984
    PubMed
    Summary

    Ethylmethanesulphonate (EMS) treatment of Amoeba indica can produce viable mini amoeba cells, particularly when applied during the early S phase. These mini amoebae exhibit stable, altered characteristics, suggesting a cell-cycle-specific origin for this size mutant.

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    Area of Science:

    • Cell Biology
    • Mutagenesis
    • Developmental Biology

    Background:

    • Ethylmethanesulphonate (EMS) is a chemical mutagen used to induce genetic alterations.
    • The cell cycle comprises distinct phases (G1, S, G2, M) with varying sensitivities to mutagens.
    • Amoeba are single-celled organisms widely used in biological research.

    Purpose of the Study:

    • To investigate the effect of EMS treatment on Amoeba indica at different cell cycle phases.
    • To characterize the resulting mini amoeba cells and assess their viability and stability.
    • To understand the relationship between DNA's functional state and mutagenesis.

    Main Methods:

    • Treatment of Amoeba indica with ethylmethanesulphonate (EMS) at specific cell cycle phases (early S, late S, late G2).
    • Observation and characterization of mini amoeba cells produced post-treatment.
    • Analysis of cell size, structure, membrane properties, cell-cycle timing, and macromolecular synthesis patterns.

    Main Results:

    • EMS treatment induced mini amoeba formation, primarily in the G2 phase.
    • Mini amoebae derived from early S phase EMS treatment showed higher viability and formed stable clones.
    • These viable mini amoebae displayed stable, altered characteristics compared to parental cells.

    Conclusions:

    • The mini amoeba is a size mutant with a cell-cycle-phase-specific origin.
    • Early S phase appears to be a critical window for generating viable, stable mutants with EMS.
    • Preferential mutagenic action on functionally distinct DNA states may lead to viable mutant amoebae.

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