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Polymeric columns for liquid chromatography.

J R Benson, D J Woo

    Journal of Chromatographic Science
    |September 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Modern polymeric columns offer high efficiency and reproducibility in liquid chromatography. They are ideal for separating ionic species, amino acids, and proteins, overcoming limitations of traditional silica-based columns.

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    Area of Science:

    • Analytical Chemistry
    • Polymer Science

    Background:

    • Polymeric columns are increasingly used in liquid chromatography due to material advancements.
    • Older polymeric columns had limitations, but modern versions offer improved rigidity and efficiency.

    Purpose of the Study:

    • To highlight the advantages and applications of modern polymeric columns in liquid chromatography.
    • To compare the performance of polymeric columns with traditional silica-based columns.

    Main Methods:

    • Advancements in polymer synthesis and material properties.
    • Evaluation of polymeric column performance in various chromatographic applications.

    Main Results:

    • Modern polymers exhibit high rigidity, enabling faster analyses at normal eluent flow rates.

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  • Polymeric columns achieve efficiencies comparable to or exceeding silica materials (over 100,000 plates/m).
  • Polymers demonstrate superior performance in separating ionic species, amino acids, peptides, proteins, organic acids, carbohydrates, and inorganic ions compared to silica.

    • Conclusions:

    • Polymeric columns provide a robust alternative to silica for challenging separations, especially those involving aqueous eluents and ionic species.
    • Exceptional lot-to-lot reproducibility of polymeric columns addresses a key limitation of silica packings.