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Related Experiment Videos

Cellular changes with graded limb ischemia and reperfusion.

M O Perry, G T Shires, S A Albert

    Journal of Vascular Surgery
    |July 1, 1984
    PubMed
    Summary
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    This study shows that muscle cell membrane potential difference decreases significantly during ischemia and reperfusion, even when energy levels are normal. This suggests membrane potential is a sensitive indicator of cell dysfunction in ischemia.

    Area of Science:

    • Biomedical Engineering
    • Physiology
    • Cell Biology

    Background:

    • Ischemia, a condition of reduced blood flow, can lead to cell damage.
    • Assessing cell membrane function is crucial for understanding ischemia's effects.

    Purpose of the Study:

    • To investigate the impact of ischemia and reperfusion on muscle cell membrane potential difference (PD).
    • To compare the sensitivity of PD measurements versus metabolite levels in detecting ischemia-induced cell dysfunction.

    Main Methods:

    • Inducing ischemia in canine hind limbs by reducing arterial pressure.
    • Measuring muscle membrane potential difference (PD), blood gases, and metabolites at baseline, during ischemia, and after reperfusion.

    Main Results:

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  • Muscle creatine phosphate decreased, but adenosine triphosphate remained normal during ischemia.
  • Muscle membrane potential difference (PD) significantly decreased during ischemia and further after reperfusion.
  • PD deterioration persisted despite normal intracellular energy levels.

    • Conclusions:

    • Muscle membrane potential difference (PD) is a sensitive indicator of ischemia-induced cell dysfunction.
    • Cell membrane dysfunction during ischemia may not correlate with intracellular energy status.
    • Oxygen free radicals may contribute to the observed decline in membrane potential.