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Updated: Jul 15, 2026

A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli
Published on: December 9, 2017
Polymorphic acetylation of 7-amino-clonazepam in human liver cytosol
Abstract:
The N-acetylation of the reduced metabolite of clonazepam 7-amino-clonazepam was studied in cytosolic preparation from human fetal and adult livers. The metabolite formed 7-acetamido-clonazepam was measured with high performance liquid chromatography. A bimodal distribution of the N-acetyltransferase activities was observed in cytosols from human adult livers. These activities were 117 +/- 11 and 27 +/- 16 pmoles X mg-1 X min-1 for rapid and slow acetylators, respectively. The data observed in the fetal specimens did not allow any conclusion about bimodality because of a low number of samples.
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