Related Experiment Video
Updated: Jul 17, 2026
11:06
Method for the Isolation of Francisella tularensis Outer Membranes
Published on: June 30, 2010
Isolation and purification of moniliformin.
Journal - Association of Official Analytical Chemists
|May 1, 1978
Summary
This study details a bulk purification method for moniliformin, a mycotoxin from Fusarium moniliforme. The developed procedure yields purified moniliformin for further toxicological and analytical studies.
Area of Science:
- Mycology
- Toxicology
- Analytical Chemistry
Background:
- Fusarium moniliforme is a common fungal pathogen.
- Moniliformin is a mycotoxin produced by this fungus.
- Accurate quantification and toxicological studies require pure mycotoxin standards.
Purpose of the Study:
- To develop an efficient bulk purification protocol for moniliformin.
- To characterize the purified mycotoxin.
Main Methods:
- Methanol extraction of contaminated maize.
- Aqueous extraction of the residue.
- Ion exchange chromatography with a NaCl gradient.
- Desalination and crystallization.
Main Results:
- A scalable purification method for moniliformin was established.
- The pKa of moniliformin was determined to be 1.70.
- Molar absorptivities were reported in aqueous and methanolic solutions.
Conclusions:
- The described method provides a reliable source of purified moniliformin.
- This facilitates further research into its toxicology and detection.
- The characterization data aids in analytical method development.
