Comparisons of different sampling designs for the determination of genetic transmission mechanisms in quantitative...

The power provided by several sampling designs to detect segregation at a major locus was investigated in a simulation study using phenotypes constructed from a major-locus genotypic mean, a background polygenic effect, and an individual-specific environmental effect. Questions of which relatives, how many relatives, and how many independent pedigrees to collect were considered, using configurations ranging from nuclear families of size 5 to 4-generation pedigrees of size 45. Each configuration contained a single proband whose phenotype exceeded the 95th percentile in a population where 2.5% carry the disease susceptibility allele. Results suggest that, under the conditions simulated, when total sample size is fixed, samples composed of 3-generation pedigrees of intermediate size provide a greater magnitude of support for the presence of a major locus than do samples composed of nuclear families or 4-generation pedigrees. This study is the first to consider both the discriminatory power and estimation efficiency in comparing alternative sampling strategies for pedigree data.