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N-malonyltransferases from peanut.

U Matern, C Feser, W Heller

    Archives of Biochemistry and Biophysics
    |November 15, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Researchers identified three distinct N-malonyltransferases in peanut seedlings, crucial for metabolizing various compounds. These enzymes play a role in plant defense and transport, with some conjugates potentially metabolized in older plants.

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    Area of Science:

    • Plant biochemistry
    • Enzymology
    • Xenobiotic metabolism

    Background:

    • N-malonic acid conjugates are found in peanuts, either naturally or after exposure to foreign compounds (xenobiotics).
    • Understanding the enzymes involved in conjugate formation and breakdown is key to plant metabolic studies.

    Purpose of the Study:

    • To purify and characterize N-malonyltransferases from peanut seedlings.
    • To investigate the substrate specificity and potential roles of these enzymes in peanut metabolism.

    Main Methods:

    • Purification of three distinct N-malonyltransferases from peanut seedlings.
    • Enzyme assays using various substrates like anthranilic acid, D-tryptophan, and 3,4-dichloroaniline.
    • Distinguishing N-malonyltransferases from O-malonyltransferase activities.

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  • Hydrolysis assays using crude and dialyzed plant extracts.

    • Main Results:

    • Three specific N-malonyltransferases were isolated, each accepting different substrates.
    • One enzyme also conjugated 1-aminocyclopropane-1-carboxylic acid.
    • N-malonyltransferases were distinct from O-malonyltransferase activities.
    • Young seedlings' extracts could not hydrolyze conjugates, but older plants' extracts showed some hydrolysis.

    Conclusions:

    • Peanut seedlings possess multiple N-malonyltransferases with distinct substrate specificities.
    • These enzymes may be involved in vacuolar transport and detoxification of xenobiotics.
    • Metabolism of some N-malonic acid conjugates appears to occur in senescing peanut plants.