3H-2-deoxyglucose uptake after electrical stimulation of cardioactive sites in anterior medial cortex in rabbits

The anterior medial cortex is an important integrative area for cardiovascular adjustments occurring during learning and conditioning. The autoradiographic 3H-2-deoxyglucose (3H-2DG) method for regional cerebral metabolic activity was used to identify other forebrain regions associated with cardiovascular adjustments elicited by electrical stimulation of anterior medial cortex. Rabbits that received anterior midline stimulation that produced bradycardia and depressor responses showed increased metabolic activity in the ipsilateral mediodorsal (MD) nucleus of the thalamus and the dorsal aspect of the claustrum. Two additional animals with anterior cortical placements in the infralimbic and anterior limbic areas showed ipsilateral increased activity in perirhinal cortex, but more dorsolateral placements in the precentral agranular area did not produce increased perirhinal activity. Control animals did not show this pattern of activity. These data suggest that MD and claustrum participate in a neural circuit which mediates cardiovascular adjustments similar to those elicited by Pavlovian conditioning contingencies.