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Related Experiment Videos

Are immunoassays for digoxin reliable?

S J Soldin, A Papanastasiou-Diamandi, J Heyes

    Clinical Biochemistry
    |October 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

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    Radioimmunoassay and fluorescence polarization immunoassay procedures can yield false positive digoxin results due to interfering steroids and lipids. Careful interpretation of immunoassay digoxin measurements is recommended.

    Area of Science:

    • Clinical Chemistry
    • Analytical Chemistry
    • Pharmacology

    Background:

    • Digoxin is a critical medication for heart conditions.
    • Accurate digoxin measurement is essential for therapeutic drug monitoring.
    • Immunoassay methods are widely used for digoxin quantification.

    Purpose of the Study:

    • To assess the specificity of immunoassay methods for digoxin measurement.
    • To identify potential interfering substances in digoxin assays.
    • To evaluate the reliability of current digoxin immunoassay procedures.

    Main Methods:

    • Radioimmunoassay (RIA) procedures were utilized.
    • Fluorescence polarization immunoassay (FPIA) procedures were employed.
    • Potential interfering substances including steroids and lipids were tested.

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    Main Results:

    • Significant interference was observed from various steroids.
    • Lipids also demonstrated interference in digoxin assays.
    • False positive digoxin results were obtained under study conditions.

    Conclusions:

    • Immunoassay methods for digoxin are susceptible to interference.
    • Steroids and lipids can compromise the accuracy of digoxin measurements.
    • Clinical interpretation of immunoassay-derived digoxin levels requires caution.