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A simple microtitration technique for quantitating total haemolytic complement levels.

S T Ellis, R L Clancy

    Journal of Immunological Methods
    |January 1, 1978
    PubMed
    Summary
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    A new microtitration technique accurately measures total hemolytic complement levels in human serum. This fast and reproducible method is suitable for routine clinical screening of complement activity.

    Area of Science:

    • Clinical immunology
    • Biochemical analysis

    Background:

    • Complement system plays a crucial role in immune response.
    • Accurate measurement of complement levels is vital for diagnosing various diseases.

    Purpose of the Study:

    • To describe a simple microtitration technique for determining total hemolytic complement levels in human serum.
    • To provide a reliable method for routine clinical screening of complement activity.

    Main Methods:

    • Microtitration technique.
    • Determination of total hemolytic complement levels.
    • Analysis of human serum samples.

    Main Results:

    • The described method is fast and reproducible.
    • The technique allows for efficient screening of total complement activity.

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    Conclusions:

    • The microtitration technique is a valuable tool for clinical medicine.
    • This method offers a practical approach for assessing complement levels in patients.