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Related Experiment Videos

A method for measuring cyto-adherence on frozen tissue sections.

P J Sheldon, F Rodgers, H Walker

    Journal of Immunological Methods
    |January 1, 1978
    PubMed
    Summary
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    A new method quantifies cells on mouse spleen frozen sections using microscopy and a planimeter. This straightforward technique helps determine optimal binding conditions for specific antibodies.

    Area of Science:

    • Immunohistochemistry
    • Cell Adhesion Assays
    • Microscopy Techniques

    Background:

    • Quantifying cell adhesion to tissue sections is crucial for understanding immune responses and antibody binding.
    • Existing methods can be complex or lack reproducibility.

    Purpose of the Study:

    • To describe a simple, reproducible method for quantifying cell adherence to mouse spleen frozen sections.
    • To apply this method to investigate optimal binding conditions for specific antibodies.

    Main Methods:

    • Utilized a microscope with a drawing tube attachment and a planimeter for cell quantification.
    • Applied the technique to assess binding conditions for E-sheep and E-oxAC antibodies on mouse spleen sections.

    Main Results:

    Related Experiment Videos

    • A straightforward and reproducible method for quantifying adherent cells was established.
    • Preliminary data on optimal binding conditions for E-sheep and E-oxAC were obtained.

    Conclusions:

    • The described method offers a simple and reliable approach for cell adhesion quantification on frozen tissue sections.
    • This technique facilitates the optimization of antibody binding conditions in immunological studies.